The name is derived from allantoin, a product of uric acid excreted by the fetus into the allantois, and oxaluric acid derived from oxalic acid and urea, found in urine. This study was to create a longterm rabbit model of diabetes mellitus for medical studies of up to one year or longer and to evaluate the effects of chronic hyperglycemia on damage of major organs. Transarterial wall oxygen gradient measurements were performed on the infrarenal aorta with an oxygen microelectrode 14 to 16 weeks after the induction of alloxan diabetes in rabbits. These effects are in partly due to its potent antioxidant properties. Total antioxidant capacity, nutritional composition and. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Six rabbits showed elevated blood glucose levels, and were distributed into 3 experimental groups groups 1, 2, and 3, each comprising 2 rabbits with dm. The effect of chronic alloxan and streptozotocininduced diabetes on isolated rat heart performance. Conventional drugs treat diabetes by improving insulin sensitivity, increasing insulin production andor decreasing the amount of glucose in blood. The first group 5 rabbits was used as control and fed with standard chow and tap water. The alloxan model of diabetes was first described in rabbits by dunn, sheehan and mcletchie in 1943. Histopathological abnormalities of prolonged alloxan.
Rabbits were weighed weekly throughout the study and the weights were recorded. To study the effect of mogroside extract on splenic lymphocytes subsets and the cytokines expression levels of type 1 diabetes mellitus tidm mice. Diabetes mellitus was induced by a 120 mgkg body weight intravenous injection of alloxan. Alloxaninduced diabetes, a common model for evaluating the. Effect of haematogenous oxidation therapy with ul travioletc. Diabetes was induced in the experimental rabbits with alloxan 35 mgkg body weight. Heart histopathology and oxidative features in diabetic. Several herbal preparations are used to treat diabetes, but their reported hypoglycemic effects are complex or even paradoxical in some cases. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds.
In the present investigation the pathological effects of alloxan induced acute hypoglycaemia were studied in rabbits. Optimization of alloxan dose is essential to induce stable. Effect on liver enzymes due to voglibose in alloxaninduced. This study aimed to create diabetic animal models and to provide an exact and detailed account of alloxan induced diabetes in rabbits.
Another 12 agematched normal rabbits were used as controls. Alloxaninduced diabetes mellitus how is alloxaninduced. Genetic and surgical models were rarely used because they required highly technical skills and the percentage of animals lost during the procedures were higher. The national center for biotechnology information us national library of medicine. Alloxan has been administered in single or multiple. In 1937 jacobs 1 found that alloxan 2 the ureide of mesoxalic acid produced fatal hypoglycemia when injected intravenously into rabbits. Similar well established granulomata in rabbits with acute alloxan diabetes induced 8, 10, and 15 days after.
Blood glucose lowering activity of aloe based composition. Balbc mice were rendered diabetic by a single intraperitoneal injection of alloxan, at dose of 200 mgkg bw. In recent years, the popularity of medicinal plants as a remedy has been increased manifold due to having minimal adverse effects. By continuing to use our website, you are agreeing to our use of cookies. However, the underlying mechanisms are still not clearly elucidated. Pathomorphological effects of alloxan induced acute. On microscopic study these observers found necrosis of. Alloxaninduced diabetes, a common model for evaluating. Blood glucose levels were monitored 0 h, 20 min, 1 h, and then hourly up to 5 h and clinical signs noted. The aim of this study was to evaluate the atrial electromechanical function, atrial electrophysiological changes and af inducibility in alloxan induced diabetic rabbits. In 14 rabbits previously injected intravenously with alloxan dose 75150 mgkg with subsequent hyperglycaemia, intraarteriolar aggregation of platelets at the sites of small standardized electrical injuries to cerebral cortical vessels showed an increased sensitivity p is less than 0. Diabetes was induced using alloxan monohydrate 80 mgkg. Background the present study described the phytochemical profile of lavandula stoechasessential oils, collected in the area of aindraham northwest of tunisia, as well as their protective effects.
We report herein the findings of this research work. Research article alloxan induced diabetes mellitus in wistar. Similar well established granulomata in rabbits with acute alloxan diabetes induced 8, 10, and 15 days after injection of the fungus uniformly showed activation of the infection. Chronic diabetes mellitus was induced in rats with alloxan monohydrate. Evaluating the invivo effects of methanolic extract of a. However, the in vivo effects of oxytocin on marrow adipogenesis in diabetes remain unknown. Glycosuria persisted for the 6 weeks of study, but ketonuria was never encountered. Anion gap toxicity in alloxan induced type 2 diabetic rats. Oxytocin treatment prevents marrow adiposity observed in. Fortyfive female new zealand rabbits were randomly divided into controls, diabetes, and diabetes treated with oxytocin ip, 0. Oxytocin might be used therapeutically as an ally to rescue osteopathy resulting from diabetes. Only alloxanised hyperglycemic and glycosuria animals were used for further. Alloxan induced diabetic rabbits as animal model for diabetes. Coronary artery ligation and reperfusion in alloxan.
The present study was undertaken with the object of determining whether the progression of cataract formation, when the latter is induced by diabetes, might be altered by the following procedures. The effects of alloxan induced diabetes has been demonstrated to be due to oxidative damage following the generation of superoxide radicals as a result of increased xanthine oxidase production, an enzyme involved in the generation of superoxide radicals. In this longitudinal study, we aimed to investigate the protective effects of oxytocin on diabetesinduced marrow adiposity in rabbits using proton mr spectroscopy. The current study aimed to compare the hypoglycemic, hypolipidemic and hepatoprotective effects of the ethanolic extract of asparagus racemosus eear linn. Alloxan monohydrate is a common drug used for developing experimental diabetes in animals, as streptozotocin, though less toxic than alloxan is considerably costlier. Dec 28, 20 subacute essential oils treatment induced a decrease of lipoperoxidation as well as an increase of antioxidant enzyme activities. Cataracts induced by alloxan diabetes in rats and rabbits. Phytochemical screening, antihyperglycemic and antihyperlipidemic effects of aqueous extracts of l. Recently, a dramatically increasing prevalence brought diabetes mellitus and its therapy to the focus of public health interests in bangladesh.
Frontiers antihyperglycemic and antihyperlipidemic effects. Free fulltext pdf articles from hundreds of disciplines, all in one place hypoglycemic effect of trigonelline isolated from iraqi fenugreek seeds in normal and alloxandiabetic rabbits pdf paperity. These findings suggested that lavandula stoechas essential oils protected against diabetes and oxidative stress induced by alloxan treatment. Boonei on liver enzyme function in alloxan induced diabetes mellitus. Subacute essential oils treatment induced a decrease of lipoperoxidation as well as an increase of antioxidant enzyme activities. Alloxan induces damage and death of pancreatic isletcells in several experimental animal models, thus causing diabetes mellitus and decreasing the secretion of insulin 31. Increased sensitivity of in vivo platelet aggregation in. Alloxan exerts its diabetogenic action when it is administered parenterally. Cuiidependent dna damage increased with the concentration of alloxan and nadh. Frontiers antihyperglycemic and antihyperlipidemic. Green tea, alloxaninduced diabetic rabbits, antidiabetic and weight regaining effect, hyperglycemiainduced, solvent fractions. The antioxidant composition of unripe plantain and its free radical scavenging activity on alloxan induced diabetic rabbits and on dpph radical was investigated. Green tea, alloxan induced diabetic rabbits, antidiabetic and weight regaining effect, hyperglycemia induced, solvent fractions. European scientific journal august 2014 special edition issn.
The mechanism of dna damage induced by alloxan was investigated using 32plabeled human dna fragments. Similar experiments conducted by dunn, sheehan and mcletchie 3 confirmed the hypoglycemic action of alloxan. The mechanisms of alloxan and streptozotocininduced diabetes. The usage of medicinal plants is traditionally rooted in bangladesh and still an essential part of public healthcare. Keywords diabetes mellitus, asparagus racemosus, gliclazide, pioglitazone, combination therapy 1. Rabbits are increasingly being used as experimental diabetic models and alloxan has been the chemical of choice for the development of. The peak values for blood sugar were on the sixth week of. The use of nonpharmacological treatments herbal agents is a new approach in the management of diabetes.
The basal glucose level of the animals was measured before they were induced to have type 2 diabetes. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Pathomorphological effects of alloxan induced acute hypoglycaemia. Alloxan diabetes alters the rabbit transarterial wall oxygen. Diabetes mellitus was induced by injection of alloxan 120 mgkg body weight, via the marginal ear vein. It was due to the stimulatory effect of strychnine alkaloid on the intestinal l cells which further produced high concentrations of glucagon like peptide hormone1 glp1. Quadriceps muscle from rabbits 16 wks after onset of diabetes and hyperglycemia were analyzed with biochemical and in situ methods. Olive leaves extract compounds, such as oleuropein, have been used in many therapeutic and preventive cases. The present paper is conducted to test the hypoglycemic and antioxidative activities of the leaf essential oil of p. Diabetes induced arterial wall hypoxia is proposed as an initiator and continuing stimulus for atherosclerotic vascular occlusive disease.
The blood glucose level of diabetic rabbits increased significantly throughout the experimental period. Metformin was orally administered at 50 mgkg, while the extract was administered both to normal and diabetic rabbits at 100, 200 and 300 mgkg. Introduction diabetes mellitus dm is the most widespread chronic disease of the present decade which is associated with absolute or relative deficiency of circulating in. The effects of aqueous extract of alfalfa on blood glucose. The ethanol extract of fenugreek seeds significantly decreases the plasma malondialdehyde mda from 2.
Diabetes follows the alloxaninduced destruction of the. What is the right dose of alloxan to induce diabetes 1 in. What is the right dose of alloxan to induce diabetes 1 in albino rats. Histopathological abnormalities of prolonged alloxaninduced. We conducted an ethnobotanical survey to identify the traditional medicinal plants being used to. Since then, alloxan diabetes has been commonly utilized as an animal model of insulindependent diabetes mellitus iddm. Diabetes was produced in rabbits by injection of the toxic glucose analogue alloxan, which selectively enters pancreatic beta cells and irreversibly decreases insulin production, similar to streptozotocin. Alloxan is known to induce diabetes in experimental animals through destruction of insulinproducing 3cells of pancreas. Embelia ribes er has been documented in ayurveda for treating various diseases, including diabetes mellitus dm. Pdf creating a longterm diabetic rabbit model researchgate. The results showed a significant improvement in the stress induced oxidation parameters by alloxaninduced diabetes in rabbits. There are several therapeutic options for the treatment of diabetes but adequate glycemic. Garlic in alloxaninduced diabetic rabbits the american.
Hypoglycemic and antioxidant effects of leaf essential oil. It has been successfully induced in a variety of animal species. Alloxan induced diabetes consistently produced the main characteristics of diabetes mellitus including polydipsia, polyphagia, polyuria, decreased insulin levels, weight loss and hyperglycemia. I am online and ready to help you via whatsapp chat. In view of the inhibitory effect on the development of experimental cholesterol atherosclerosis observed in alloxan diabetic rabbits, the effect of alloxan diabetes on the retrogression of such arterial lesions was studied in another series of experiments. The mechanism of alloxan and streptozotocin action in b cells. Traditional medicinal plants used for the treatment of. They stated that the this dose of alloxan agents required for inducing dm can be toxic if it administrate by the route of intravenous iv 5, 12. The aim of this study was to evaluate the atrial electromechanical function, atrial electrophysiological changes and af inducibility in alloxaninduced diabetic rabbits. Coronary artery ligation and reperfusion in alloxandiabetic rabbits. After confirmation of tidm, control and diabetic mice were further subdivided into the following six. Literature search was performed in pubmedmedline, embase, scopus, sciencedirect.
May 24, 2014 thus, it allows elucidation of antihyperglycemic agents in the treatment of diabetes. Dec 18, 2007 alloxan has two distinct pathological effects. Sir, we read with interest the article titled anomalies in alloxan induced diabetic model. Determination of the optimal diabetes duration for bone. A single dose of alloxan monohydrate 100 mgkg was given intravenously to 20 young new zealand white rabbits. The most frequently used intravenous dose of this drug to induce diabetes in rats is 65 mg kg1 body weight. Effect of alloxan diabetes upon the phagocytic and bactericidal efficiency of rat leukocytes for pneumococcus. Get a printable copy pdf file of the complete article 4.
Hypoglycemic herbs and their action mechanisms chinese. In view of the inhibitory effect on the development of experimental cholesterol atherosclerosis observed in alloxandiabetic rabbits, the effect of alloxan diabetes on the retrogression of such arterial lesions was studied in another series of experiments. Effect of haematogenous oxidation therapy with ultravioletc irridation in alloxaninduced diabetes and poloxamer 407induced hyperlipidemia in rabbits. For full access to this pdf, sign in to an existing account, or purchase. When they finish that diet, ordinary food was given to those. Pdf this study was to create a longterm rabbit model of diabetes. It is better to standardize it first by jain et al. In remaining ten rabbits, diabetes mellitus was induced by alloxan monohydrate 150 mgkg by intraperitoneal administration. The mechanism of alloxan and streptozotocin action in b. Diabetes was confirmed after 3 days of alloxan injection by determining the fasting blood glucose concentration. Effect of haematogenous oxidation therapy with ultravioletc irridation in alloxan induced diabetes and poloxamer 407 induced hyperlipidemia in rabbits. The mechanisms of alloxan and streptozotocin induced diabetes. Alloxan rapidly and selectively accumulates in pancreatic beta cells gorus et al. Oral administration of dahi containing probiotic lactobacillus acidophilus and lactobacillus casei delayed the progression of streptozotocininduced diabetes in rats volume 75 issue 2 hariom yadav, shalini jain, pushpalata ravindra sinha.
In fact, diabetes is a chronic metabolic disease, which is associated with oxidative stress and hyperglycaemia which have direct toxic effects on cardiomyocytes. Diabetes induced cardiac dysfunction and lesions are nowadays confirmed. The effect of pregnancy complicated by alloxan diabetes on the fetuses of dogs, rabbits and rats, endocrinology, volume 40, issue 4, 1 april 1947. Impaired atrial electromechanical function and atrial. Diabetic atherosclerosis was induced in rabbit iliofemoral arteries by. Altered glucose metabolism and hypoxic response in alloxan. Modulation of hematological indices of normal and alloxan. In normal rabbits subcutaneous granulomata produced by the injection of a spore suspension of rhizopus oryzae remained confined to the site of inoculation, showed no fungus proliferation, no longer yielded the agent on culture 10 weeks after inoculation, and eventually healed. Alloxan induced diabetes in this, however, remains contradictory to many studies where alloxan has xlloxan successfully used for the same.
Department of veterinary physiology, biochemistry and pharmacology, college of medicine, university of ibadan. Alloxan is a cyclicurea derivative, which is a highly effective, diabetogenic agent used widely as a tool to induce experimental diabetes. The aim of this study was to investigate the effect of aqueous extract of alfalfa on blood glucose and serum lipids in alloxaninduced diabetic rats. Of the 22 experimental rabbits, 9 died within 7 days, 5 failed to show elevated blood glucose levels, and 2 died during bone defect surgery. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Diabetes mellitus dm is an independent risk factor for atrial fibrillation af. Jain and arya in 2011 state that doses of drugs, routes of administration, are more important issue in duration and severity of diabetes and methodology in alloxaninduced diabetic models. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and. Research article alloxan induced diabetes mellitus in. Garlic in alloxaninduced diabetic rabbits, the american journal of clinical nutrition, volume 28, issue 7, 1 july 1975, pages 684685, htt we use cookies to enhance your experience on our website.
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